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为研究哺乳期暴露阿特拉津对仔鼠精原细胞凋亡的影响,将16窝健康清洁级昆明小鼠[16只母鼠及出生后7 d雄性仔鼠(每窝2～5只)]随机分为4组,分别为对照(植物油)组和低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量阿特拉津染毒组,每组4窝。对母鼠进行灌胃染毒,染毒剂量为10 ml/kg,每天1次,连续进行8 d。仔鼠于生后7～15 d通过母乳染毒阿特拉津。染毒结束后,测定仔鼠睾丸生精管中精原细胞的凋亡情况。结果显示,与对照组比较,各剂量阿特拉津染毒组仔鼠精原细胞凋亡率增高,差异均有统计学意义(P<0.05,P<0.01);且随着阿特拉津染毒剂量的升高,仔鼠精原细胞凋亡率呈上升趋势。表明阿特拉津可以通过母鼠乳汁增高仔鼠精原细胞的凋亡率,影响精子的发生。
In order to study the effect of atrazine on the apoptosis of spermatogonial cells during lactation, 16 healthy and clean Kunming mice (16 males and 7 days postnatal male offspring (2 ~ 5) ] Were randomly divided into 4 groups: control (vegetable oil) group and atrazine (50 mg / kg), middle (100 mg / kg) and high (200 mg / nest. Gavage on the mother rats, exposure dose of 10 ml / kg, once daily, for 8 days. Offspring 7 to 15 days after birth by atrazine exposure to atrazine. After the exposure, the apoptosis of spermatogonia in testis voles was determined. The results showed that compared with the control group, the apoptotic rate of spermatogonia in offspring of each dose of atrazine increased, the differences were statistically significant (P <0.05, P <0.01); and with atrazine With the increase of dose, the apoptotic rate of spermatogonia in offspring increased. It shows that atrazine can increase the apoptosis rate of spermatogonia in the offspring of maternal mice and affect the spermatogenesis.