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建立柱前衍生化高效液相色谱法测定人血浆卡托普利浓度。方法：分析柱为Ｗａｔｅｒｓ Ｎｏｖａ－ｐａｋ Ｃ１８。流动相为乙脯：双蒸水：磷酸（１０３： １９７： ０３）。 ＵＶ检测波长为２６０ｎｍ。用对溴苯甲酰溴化物（ｐ－ＢＰＢ）与血浆的卡托普利在常温下衍生化反应３０ｍｉｎ。经衍生化反应的血浆酸化后用乙酸乙酯提取，分离有机相并碱化之，弃去上层，再进行酸化提取后测定。内标物为苯甲酰基苯甲酸。结果：测定卡托普利的线性范围为 １０～５００ｍｇ ·Ｌ－１，最低检测浓度为５ｍｇ· Ｌ－１。日内精密度＜８．２７％，日间精密度＜７．７０％。方法回收率均＞９０％。结论：本方法精密、准确，适用于卡托普利的药代动力学研究，也可用于测定尿中卡托普利浓度。
To establish a precolumn derivatization high performance liquid chromatographic method for the determination of captopril concentration in human plasma. Method: Analytical column Waters Nova-pak C18. The mobile phase is B: double distilled water: phosphoric acid (103: 197: 03). UV detection wavelength of 260nm. Derivatization with p-bromobenzoyl bromide (p-BPB) and plasma captopril at room temperature for 30 min. The derivatized plasma was acidified and extracted with ethyl acetate. The organic phase was separated and alkalinized. The upper layer was discarded and the residue was acidified and extracted. The internal standard is benzoyl benzoic acid. Results: The linear range of captopril was 10 ~ 500 mg · L-1 and the lowest concentration was 5 mg · L-1. Intraday precision <8.27%, day precision <7.70%. The recovery rate of the method was> 90%. Conclusion: The method is accurate and accurate, suitable for the study of captopril pharmacokinetics, also can be used to determine the concentration of captopril in urine.