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寡聚核苷酸在不同的介质条件下可形成双链、三链、四链等多种不同结构 ,它们都能与溴乙锭发生相互作用使其荧光强度发生变化 .通过荧光光谱和静态荧光技术的研究发现这些相互作用存在着不同的作用机制 ,其中B型双链和三链与溴乙锭的作用主要是一种嵌入式的 ,能量转移效率较高 ,荧光强度增加较大且不易被猝灭剂猝灭 ;平行双链与溴乙锭的作用主要是一种外挂式的 ,能量转移效率极低 ,荧光强度和猝灭常数几乎与自由溴乙锭相等 ;而四链与溴乙锭的作用机制较为复杂 ,从能量转移和荧光猝灭实验上看可能同时存在上述两种方式
Oligonucleotides can form double-stranded, triple-stranded, four-stranded and many different structures under different media conditions and all of them can interact with ethidium bromide to change the fluorescence intensity.Using fluorescence spectroscopy and static fluorescence The study found that these interactions have different mechanisms of action, in which the role of B-type and three-chain and ethidium bromide is mainly an embedded, high energy transfer efficiency, the fluorescence intensity increases greatly and is not easy to be Quench agent quenching; parallel double-stranded and ethidium is mainly a plug-in type, the energy transfer efficiency is very low, the fluorescence intensity and quenching constant almost equal to free ethidium bromide; and four chain and ethidium bromide The mechanism of action is more complicated, energy transfer and fluorescence quenching experimental point of view there may be the above two ways