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以不同等电点的明胶为原料,用浊度分光光度法和毛细管等电点聚焦法测定了它们的等电点,用乳化法在归一化的介质酸碱度下制备了一系列微球,考察了明胶等电点对所制得微球的分散度的影响。结果表明,等电点越接近溶液pH值的明胶,制得的微球直径越小,分布越均匀,球形和流动性越好;等电点偏离溶液pH值越远的明胶,制得的微球直径越大,分布越不均匀,球形和流动性越差。因此,乳化法制备明胶微球时应该把溶液pH值调至尽可能接近所用明胶的等电点值。这是微球设计和制备必须遵循的一条重要准则。
Using isoelectric point gelatin as raw material, their isoelectric points were determined by turbidimetric spectrophotometry and capillary isoelectric focusing, and a series of microspheres were prepared by emulsification under the normalized medium pH. The effect of gelatin isoelectric point on the dispersion of microspheres prepared. The results show that the closer the isoelectric point is to the gelatin of the solution pH, the smaller the diameter of the prepared microspheres is, the more uniform the distribution is, the better the spherical shape and fluidity; the gelatin with the isoelectric point deviates from the pH value of the solution, The larger the ball diameter, the more uneven the distribution, the poorer the sphere and fluidity. Therefore, when gelatin microspheres are prepared by emulsification, the solution pH should be adjusted to be as close as possible to the isoelectric point of the gelatin used. This is an important guideline that microspheres must follow to design and prepare.