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[目的]研究亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性及其与烟酒茶嗜好相互作用与贲门癌易感性的关系。[方法]在上消化道癌高发区淮安市进行了病例-对照研究(贲门癌89例,对照人群223例),调查研究对象的生活习惯,采用PCR鄄RFLP技术检测研究对象的MTHFR基因型。应用SAS软件或EPIInfo软件进行统计学分析。[结果]①男性吸烟者和女性饮酒者与男性不吸烟者和女性不饮酒者相比患贲门癌的风险增高;不饮茶者与饮茶者相比发生贲门癌的危险性升高。②贲门癌组中MTHFRC/T+T/T基因型携带者占82.0%,显著高于对照组的70.3%。与携带MTHFRC/C基因型相比,C/T+T/T基因型携带者发生贲门癌的危险性(OR)为2.12(95%CI1.14～3.95),调整性别、年龄、吸烟、饮酒、饮茶及生蔬菜、水果、肉和豆制品摄取习惯后OR为2.05(95%CI1.05～2.03)。③在吸烟、每周饮酒≥2次和不饮茶者中,携带MTHFRC/T+T/T基因型者发生贲门癌的危险性显著上升。[结论]MTHFR基因型与贲门癌的易感性有关;MTHFR基因多态性影响吸烟、饮酒和饮茶与贲门癌之间的关系。
[Objective] To investigate the relationship between the C677T polymorphism of methylenetetrahydrofolate reductase (MTHFR) gene and its preference for smoking and drinking tea and the susceptibility to gastric cardia cancer. [Methods] A case-control study (89 cases of cardia cancer and 223 control subjects) was conducted in Huaian, a high incidence area of upper gastrointestinal cancer. The living habits of the subjects were investigated. The MTHFR genotype of the study subjects was detected by PCR-RFLP. Apply SAS software or EPIInfo software for statistical analysis. [Results] (1) Male smokers and female drinkers had a higher risk of cardia cancer than male non-smokers and female non-drinkers; those who did not drink tea had a higher risk of developing cardia cancer than those who drank tea. ② In the cardia cancer group, MTHFRC / T + T / T genotype carriers accounted for 82.0%, significantly higher than 70.3% of the control group. Compared with the MTHFRC / C genotype, the risk of cardia cancer was 2.12 (95% CI 1.14 to 3.95) in C / T + T / T genotype. The sex, age, smoking, alcohol consumption OR of 2.05 (95% CI 1.05 ~ 2.03) after consuming tea and raw vegetables, fruits, meat and soy products. ③ In smoking, drink more than 2 times per week and those who do not drink tea, those who carry MTHFRC / T + T / T genotype had a significant increased risk of cardiac cancer. [Conclusion] The genotype of MTHFR is related to the susceptibility of cardia cancer. The polymorphism of MTHFR gene affects the relationship between smoking, drinking, drinking tea and cardia cancer.