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从柑橘果园的堆积土中筛选出一株丝状白腐真菌,通过正交试验确定其最佳的生长环境并粗略测定其酶活力,利用该菌株对柑橘皮渣进行分解,并对分解后的柑橘皮渣进行厌氧发酵产甲烷试验.研究表明:在该菌株单因素下,25天内,该菌株对柑橘皮渣的分解率平均达到30%.正交实验结果显示,对柑橘皮渣分解最好的处理组合条件是麸皮2g、柑桔皮渣含水量60%、初期pH3、硫酸铵0.2g.产生还原糖量平均达到517μg/g,木糖120.8μg/g,纤维素+纤维素分解量0.96g,均失重达到67%,能够有效提高柑橘皮渣的pH从pH1提高到pH4.酶活力测定表明,菌丝覆盖柑桔皮渣的第8天到第26天之间,AVI的活力均要远高于CMC和FPA.AVI在培养的第13天酶活力达到最大,第17天后酶活性直激下降.其中CMC在整个培养期间几乎没有变化,一直保持平稳.对柑橘皮渣预处理后进行厌氧产甲烷发酵,试验表明,处理后的柑橘皮渣产沼气总产量较不处理前提高20%,甲烷含量提高26%,COD值都能有效地降低达到27%,发酵前后还原糖量降低20%.
A filamentous white-rot fungus was screened from the accumulated soil of citrus orchards. The optimum growth environment was determined by orthogonal test and its enzyme activity was roughly measured. The citrus peel was decomposed by this strain. Citrus peel residue for methane production by anaerobic fermentation.The results showed that under the single factor of this strain, the decomposition rate of citrus peel was up to 30% within 25 days.Orthogonal experiment showed that the decomposition of citrus peel Good combination of processing conditions is bran 2g, citrus peel 60% moisture content, the initial pH3, ammonium sulfate 0.2g produce reducing sugar an average of 517μg / g, xylose 120.8μg / g, cellulose + cellulose decomposition The amount of 0.96g, weight loss of 67%, can effectively improve the pH of citrus peel slag pH from 1 to pH 4. Determination of enzyme activity showed that hyphae covered citrus peel 8 to 26 days, AVI vitality Were much higher than that of CMC and FPA.AVI reached the maximum on the 13th day of culture, but decreased on the 17th day.Among them, CMC remained almost unchanged during the whole culture period, and citrus peel residue pretreatment After anaerobic methane fermentation, the test showed that the treated citrus peel residue Before biogas production increased by 20% compared with no treatment, increase the methane content of 26%, COD values can be effectively reduced 27%, 20% reduction in the amount of reducing sugars before and after fermentation.