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①目的探讨阿霉素对抗阿霉素细胞系Ｓ－１８０－Ｒ细胞凋亡的作用。②方法用低剂量（３．２５μｇ）和高剂量（６．５０μｇ）阿霉素分别处理亲本Ｓ－１８０和抗药Ｓ－１８０－Ｒ细胞腹腔接种的ＢＡＢＬ／Ｃ鼠，７２ｈ取腹水，用流式细胞仪测定细胞ＤＮＡ相对含量。③结果低剂量阿霉素７２ｈ使亲本Ｓ－１８０细胞出现６２．５％的凋亡，使抗药Ｓ－１８０－Ｒ仅出现１３．０％的凋亡细胞，高剂量阿霉素７２ｈ使亲本Ｓ－１８０细胞几乎全部死亡，使Ｓ－１８０－Ｒ出现２０．０％的细胞凋亡。低剂量阿霉素处理７２ｈ，亲本Ｓ－１８０细胞鼠腹腔出现大量淋巴细胞；抗药Ｓ－１８０－Ｒ细胞鼠腹腔只有少量淋巴细胞出现。高剂量阿霉素处理亲本Ｓ－１８０细胞仍保留很多淋巴细胞，其他Ｓ－１８０细胞几乎全部死亡，而抗药Ｓ－１８０－Ｒ细胞中淋巴细胞消失。④结论细胞抗凋亡是细胞抗药性的明显标志之一。抗阿霉素Ｓ－１８０－Ｒ细胞接种的小鼠腹腔内淋巴细胞的数量与阿霉素的剂量有关。
Objective To investigate the effect of doxorubicin on the apoptosis of adriamycin cell line S-180-R. 2Methods BABL/C mice were inoculated intraperitoneally with parental S-180 and drug-resistant S-180-R cells at low dose (3.25 μg) and high dose (6.50 μg) doxorubicin respectively. Ascites fluid was used for 72 hours. Cytometry was used to determine the relative cellular DNA content. 3 Results Low-dose doxorubicin 72h caused 62.5% apoptosis in parental S-180 cells, making anti-drug S-180-R only 13.0% of apoptotic cells, high-dose doxorubicin 72h to make the parent Almost all of the S-180 cells died, causing apoptosis of S-180-R by 20.0%. Low-dose doxorubicin treatment 72h, a large number of lymphocytes in the abdominal cavity of the parent S-180 cells; only a small amount of lymphocytes appeared in the abdominal cavity of mice resistant to S-180-R cells. High-dose doxorubicin-treated parental S-180 cells still retained many lymphocytes, almost all of the other S-180 cells died, and lymphocytes disappeared in the resistant S-180-R cells. 4 Conclusion The anti-apoptosis of cells is one of the obvious signs of cell resistance. The number of intraperitoneal lymphocytes in mice inoculated with doxorubicin S-180-R cells was related to the dose of doxorubicin.