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本文报道应用稀土元素处理甘蔗(Soccharum of ficinarum L.)叶片,分析测定了硝态氮、氨态氮、总氮、游离氨基酸组分及游离氨基酸含量、硝酸还原酶(HR)活性等变化以及NR活性与硝态氮含量的关系,研究稀土元素处理对甘蔗体内氮素同化作用的影响。结果表明,应用100～600ppm稀土元素处理甘蔗叶片均能不同程度地减少其硝态氮含量,提高其氨态氮、总氮、游离氨基酸总含量以及NR活性。但游离氨基酸组分没有发生变化。各处理浓度中以300ppm处理的效果为最显著。高浓度稀土元素处理则呈抑制效应。甘蔗叶片硝态氮含量与叶片NR活性具有平行关系,即凡硝态氮含量高,NR活性也高;凡硝态氮含量低,NR活性也低。以上结果,说明一定浓度范围的稀土元素处理能促进甘蔗的氮素同化作用。
In this paper, leaves of sugarcane (Soccharum of ficinarum L.) were treated with rare earth elements. The contents of nitrate nitrogen, ammonia nitrogen, total nitrogen, free amino acids and free amino acids, nitrate reductase (HR) Activity and nitrate nitrogen content of the study of rare earth elements on the impact of nitrogen assimilation in sugarcane. The results showed that the application of 100 ~ 600ppm rare earth elements in the treatment of sugarcane leaves can reduce the content of nitrate nitrogen and increase the content of total nitrogen, total nitrogen, free amino acids and NR activity to some extent. However, the free amino acid components did not change. The effect of 300 ppm treatment was the most significant for each treatment concentration. High concentrations of rare earth elements showed inhibitory effect. There was a parallel relationship between nitrate nitrogen content and NR activity in sugarcane leaves, that is, high nitrate content and high NR activity; low nitrate content and low NR activity. The above results show that a certain concentration of rare earth elements can promote the nitrogen assimilation of sugarcane.