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目的探讨人脐血干细胞移植后,在BALB/C小鼠肝内建立人鼠嵌合体模型的可能性。方法分离男婴脐血单个核细胞,将该细胞悬液注射至肝部分切除的雌性BALB/C小鼠肝内;以磷酸盐缓冲生理盐水(PBS)注射肝部分切除小鼠肝内为肝切除对照组,以仅作腹腔切开为空白对照组。于术后7、14天分别处死人脐血干细胞移植组、肝切除对照组和空白对照组各8只小鼠,眶底静脉丛采血检测肝功能;取肝组织原位杂交检测Y染色体(移植细胞标记)。结果术后7、14天人脐血干细胞移植组小鼠注射肝叶与非注射肝叶内可检出Y染色体阳性细胞,并可见嵌入肝板。结论人脐血干细胞移植BALB/C小鼠可以建立人鼠嵌合体动物模型。
Objective To investigate the possibility of establishing human chimerism model in the liver of BALB / C mice after human umbilical cord blood stem cell transplantation. Methods Umbilical cord blood mononuclear cells were isolated from male infants. The cells were injected into the liver of partially resected female BALB / C mice. Liver was excised from the liver of mice by phosphate buffered saline (PBS) Control group, only for celiac incision as a blank control group. At the 7th and 14th day postoperatively, 8 human umbilical cord blood stem cell transplantation group, 6 hepatectomy group and blank control group were sacrificed and blood was taken from the orbital venous plexus for detection of liver function. The Y chromosome Cell marker). Results Y chromosome positive cells were detected in liver and non-injected liver of mice injected with human umbilical cord blood stem cell transplantation group at 7 and 14 days after operation. Conclusion Human mouse chimera animal model can be established in human cord blood stem cell transplantation BALB / C mice.