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目的:通过比较幽门螺旋杆菌(Helicobacter pylori,Hp)感染的两种检测方法,探讨TaqMan探针法在诊断幽门螺旋杆菌感染中的价值。方法:抽取70例有完整病例资料的胃镜活检标本,进行Hp分型、鉴定检测,并将实验结果与临床的碳14尿素呼气试验和病理结果进行相关性分析。结果:Hp感染的两种检测方法 (TaqMan探针法和呼气实验法)一致性程度尚可(Kappa=0.550),两种方法检测的阳性率都随病理炎症程度的加重而增加,而呼气实验法检测Hp的阳性率更高(P<0.05)。但TaqMan探针法可以将Hp进行不同毒力类型的鉴定,且TaqMan探针法可区分Hp的阳性强度(1+,2+,3+),其与病理的炎症程度有较好的相关性(γ=0.564,P<0.05)。结论:TaqMan探针法可以定性地鉴定Hp,与呼气实验法有较好的一致性。TaqMan探针法可进一步定量Hp的阳性强度,更好地反映临床的病理炎症程度。
Objective: To evaluate the value of TaqMan probe in the diagnosis of Helicobacter pylori infection by comparing two detection methods of Helicobacter pylori (Hp) infection. Methods: Totally 70 cases of gastroscope biopsy specimens with complete case data were collected for Hp typing, identification and detection, and the correlation between experimental results and clinical carbon 14 urea breath test and pathological results were analyzed. Results: The consistency of the two detection methods of Hp infection (TaqMan probe method and expiratory test method) was acceptable (Kappa = 0.550). The positive rates of both methods increased with the degree of pathological inflammation, Hp gas test positive rate was higher (P <0.05). However, the TaqMan probe method can identify Hp strains of different virulence types, and the TaqMan probe method can distinguish the positive intensity (1 +, 2 +, 3 +) of Hp which has a good correlation with the pathological degree of inflammation (γ = 0.564, P <0.05). Conclusion: The TaqMan probe method can qualitatively identify Hp, which is in good agreement with the expiratory test method. TaqMan probe method can further quantify the positive intensity of Hp, to better reflect the degree of clinical pathological inflammation.