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通过构建皱纹盘鲍(Haliotis discus Hannai)cDNA文库并进行大规模测序,获得了1282条高质量的ESTs序列,对这些序列进行拼接,获得了包括650条序列在内的244组片段重叠群(Contigs)和632个单一序列(Singletons)。使用BlastX软件对876个Unigenes(Unigene为Contig和Singleton的总和)进行同源性对比,有393个获得了功能提示,占总基因数的45%。根据EST信息,采用RACE方法,首次得到皱纹盘鲍神经肽Y(neuropeptide Y,NPY)受体基因,并对其结构进行了分析。结果表明,该受体基因cDNA由1534个核苷酸组成,编码区1050bp,可以编码350个氨基酸;该多肽的分子量为39.56kda,等电点为9.57,具有7个明显的跨膜螺旋且TM7区有NPXXY模体。
By constructing a cDNA library of Haliotis discus Hannai and carrying out large-scale sequencing, 1282 high-quality ESTs were obtained, and these sequences were spliced to obtain 244 sets of contigs including 650 sequences ) And 632 singletons. Using BlastX software for homology comparison of 876 Unigenes (Unigene is the sum of Contig and Singleton), 393 obtained functional hints, accounting for 45% of the total number of genes. According to the EST information, the RACE method was used to obtain the neuropeptide Y (NPY) receptor gene of Haliotis discus hannai for the first time and its structure was analyzed. The results showed that the cDNA of this receptor consists of 1534 nucleotides and encodes a region of 1050 bp which encodes a protein of 350 amino acids. The polypeptide has a molecular mass of 39.56 kDa and an isoelectric point of 9.57 with seven obvious transmembrane helices and TM7 District has NPXXY motifs.