论文部分内容阅读
目的探索乙肝病毒(HBV)感染者HLA DRB基因频率及其与疾病的相关性。方法用PCR SSP对重庆地区汉族乙肝患者40例和正常人120名进行HLA DRB基因分型 ,结合临床病型与HBV复制状态作相关性分析。结果重庆地区汉族正常人以DR2(15)、DR5(12)、DR9 位点常见 ,乙肝患者与之比较DR3 基因频率明显增高(P<0.05) ,DR5(12)、DR7 基因频率也有增高 ,均见于慢性乙肝和肝硬化 ,相对危险度DR3 为2.4,DR7 为2.0。DR7基因多见于HBV高复制状态 ,相对危险度为2.4。结论HLA DR3 基因与重庆地区汉族慢性乙肝和肝硬化相关 ,DR7 与HBV高复制状态有关 ;免疫遗传因素参与慢性乙肝的发病机制。
Objective To explore the frequency of HLA DRB gene in patients with hepatitis B virus infection and its relationship with disease. Methods HLA-DRB genotyping was performed by polymerase chain reaction-restriction endonuclease SSP in 40 Han patients with HBV and 120 normal subjects in Chongqing. The genotypes of HBV genotypes were determined by PCR SSP. Results DR2 (15), DR5 (12) and DR9 loci were common in Han nationality in Chongqing area. The frequency of DR3 gene was significantly higher (P <0.05) and the frequencies of DR5 (12) and DR7 genes were also increased in patients with hepatitis B Found in chronic hepatitis B and cirrhosis, the relative risk of DR3 was 2.4, DR7 was 2.0. DR7 gene more common in HBV replication status, the relative risk of 2.4. Conclusions HLA DR3 gene is associated with chronic hepatitis B and cirrhosis in Han nationality in Chongqing. DR7 is associated with high replication of HBV. Immune genetic factors are involved in the pathogenesis of chronic hepatitis B.