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目的观察苦参碱微乳经皮吸收制剂和口服苦参素胶囊对小鼠四氯化碳(CCl4)肝损伤模型的保护作用,并比较两组之间的统计学差异。方法将昆明种小鼠(60只)随机分为正常对照组、模型组、苦参素胶囊阳性对照组和苦参碱微乳制剂高、中、低剂量组。阳性对照组采用苦参素胶囊内容物水溶液(200 mg·kg·1)预防性灌胃给药;苦参碱微乳制剂高、中、低剂量(200,100,50 mg·kg·1)组预防性经皮给药;采用CCl4诱导小鼠急性肝损伤模型。测定各组血清丙氨酸转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及肝组织匀浆超氧化物歧化酶(SOD)和丙二醛(MDA)的含量;取肝组织做病理学检测。结果与模型组相比,苦参碱微乳高、中剂量组和苦参素胶囊水溶液组的ALT均显著降低(P<0.05),肝组织匀浆中SOD含量明显升高(P<0.05),MDA含量明显降低(P<0.05),并减轻肝组织的病理变化。苦参碱微乳高剂量组和苦参素胶囊水溶液组的AST均显著降低(P<0.05);苦参碱微乳制剂组ALT,AST水平及SOD,MDA含量以及病理变化与苦参素胶囊水溶液组间无显著性差异。结论苦参碱微乳经皮给药对小鼠急性CCl4肝损伤具有保护作用,与临床用药苦参素胶囊比较无显著性差异。
Objective To observe the protective effect of matrine microemulsion percutaneous absorption preparation and oral oxymatrine capsule on liver injury induced by carbon tetrachloride (CCl4) in mice and compare the difference between the two groups. Methods Kunming mice (60 mice) were randomly divided into normal control group, model group, matrine capsule positive control group and matrine microemulsion medium high, medium and low dose groups. The positive control group was given prophylactic gavage with the aqueous solution of kushenin capsule (200 mg · kg · 1); the matrine microemulsion was prevented by high, medium and low dose (200,100 and 50 mg · kg · l) Transdermal administration; CCl4 induced acute liver injury model in mice. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver homogenate superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in each group. Pathological examination. Results Compared with model group, the ALT in matrine microemulsion medium dose group and matrine capsule aqueous solution group decreased significantly (P <0.05), and the content of SOD in liver tissue homogenate increased significantly (P <0.05) , MDA content was significantly lower (P <0.05), and reduce the pathological changes of liver tissue. The AST in matrine microemulsion high dose group and matrine capsule aqueous solution group were significantly decreased (P <0.05). The levels of ALT, AST, SOD, MDA and pathological changes in matrine microemulsion group were significantly lower than those in matrine capsule There was no significant difference between aqueous solution groups. Conclusion The transdermal administration of matrine microemulsion has a protective effect on acute CCl4 liver injury in mice, and there is no significant difference between the two groups.