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利用从牙鲆肠道分离的 1株鼠李糖乳杆菌P15和 1株干酪乳杆菌 ,通过试验研究其在牙鲆消化道的定植和演替规律。在投喂含有 1 2× 10 9cfu/ g乳杆菌的饲料 5d后 ,消化道定植的乳酸菌超过 10 6cfu/ g ,其后维持在 10 6～ 10 8cfu/g动态平衡中 ;同时随着乳酸菌的增加 ,2组鱼消化道的弧菌均从 10 7～ 10 8cfu/g降低到 10 5～ 10 7cfu/g。停喂乳杆菌 7d后 ,2个试验组鱼消化道的乳酸菌均从 10 5～ 10 6cfu/ g下降到 10 4～ 10 5cfu/ g ,P15组鱼小肠中弧菌从 10 5cfu/g回升到 10 7cfu/g ;LC组鱼盲囊中弧菌从 10 5cfu/g回升到 10 6cfu/g ,小肠中弧菌数量也有显著的增加。试验结果表明 ,2种乳杆菌均能在牙鲆消化道内定植 ;乳杆菌的投喂和定植 ,使牙鲆消化道中的弧菌数量明显下降
One strain of Lactobacillus rhamnosus P15 and one strain of Lactobacillus casei isolated from the intestine of Japanese flounder were used to study the colonization and succession of digestive tract in the Japanese flounder. After 5 days of feeding the feed containing 1 × 109cfu / g Lactobacillus, the number of lactic acid bacteria colonized by digestive tract was over 106cfu / g and maintained at a dynamic equilibrium of 106 ~ 108cfu / g. Meanwhile, with the increase of lactic acid bacteria Vibrio of the fish digestive tract decreased from 10 7 ~ 108 cfu / g to 105 ~ 107 cfu / g. After stopping feeding Lactobacillus for 7 days, the lactic acid bacteria in the digestive tract of fish in two experimental groups decreased from 105 to 106 cfu / g to 104 to 105 cfu / g, while the Vibrio spp. In P15 increased from 105 cfu / g to 10 7cfu / g. In the LC group, the number of Vibrio spp. Increased from 105cfu / g to 106cfu / g. The results showed that both Lactobacillus were colonized in the digestive tract of Japanese flounder; Lactobacillus feeding and colonization significantly decreased the number of Vibrio in the digestive tract of Flounder