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目的研究三维细胞培养系统(RCCS)对白色假丝酵母菌(CAL)生物学特性的影响,探讨其在模拟微重力环境下的生长规律和耐药性变化,为航天医监医保提供理论依据。方法 2014年2月采用RCCS培养CAL SC5314,试验组8株旋转瓶轴心与地面平行模拟微重力环境,对照组8株即重力组旋转瓶轴心与地面垂直;在试验第7、14和21天,分别应用分光光度计绘制菌株生长曲线、光学显微镜观察菌株形态变化、流式细胞仪检测不同浓度氟康唑(FCZ)作用下的活菌数目。结果与对照组相比,试验组CAL的生长曲线左移,迟缓期缩短,对数生长期和平台期提前,差异有统计学意义(P<0.05);试验组CAL菌丝的生成随模拟失重时相的延长而明显增加;在不同浓度FCZ作用下,试验组的活菌数较对照组明显减少,差异有统计学意义(P<0.05),并且随模拟微重力时相的延长,活菌数不断递减。结论 21d回转器模拟微重力可加速CAL的生长速度,促进菌丝生长,同时能增加菌株对FCZ的敏感性。
Objective To study the effects of three-dimensional cell culture system (RCCS) on the biological characteristics of Candida albicans (CAL) and to explore its growth and drug resistance under simulated microgravity environment, providing a theoretical basis for space medical supervision. Methods In February 2014, RCCS was used to cultivate CAL SC5314. In the experimental group, eight rotating bottle axes were simulated parallel to the ground under the micro-gravity environment. In the control group, Days, the growth curve of the strain was drawn by spectrophotometer, the morphological changes of the strain were observed by light microscope, and the number of viable cells under different concentrations of fluconazole (FCZ) was detected by flow cytometry. Results Compared with the control group, the growth curve of CAL in the experimental group shifted to the left, the lag phase shortened, and the logarithmic growth phase and plateau phase advanced significantly (P <0.05). The generation of CAL mycelium in the experimental group was associated with simulated weightlessness (P <0.05). Under the action of FCZ with different concentrations of FCZ, the number of viable cells in the experimental group was significantly decreased compared with the control group (P <0.05), and with the prolongation of simulated microgravity, Declining numbers. Conclusion 21d gyrator simulation of microgravity accelerates the growth rate of CAL and promotes the growth of mycelium, meanwhile it can increase the sensitivity of the strain to FCZ.