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本文介绍以联吡啶乙二醛单(2-吡啶基)腙(DMPH)试剂与复合维生素制剂中的钴显色产生一络合物以分光光度法测定,方法简便、正确、专属性强.测定方法:称取复合维生素片(Hidropoliuit Mineral)6片,研磨成粉,精密称取相当于3片量细粉至50ml烧杯中,加水255ml及60%高氯酸1.5ml,缓缓加热、搅拌,放冷,过滤至50ml容量瓶中,加水至刻度.精密量取滤液2.5ml至25ml容量瓶中,加10%枸橼酸氢二钠液5ml、12.5%(w/v)硫脲的二甲替甲酰胺溶液,以稀氢氧化钠溶液调节pH至4～7,加0.04%(w/v)DMPH乙醇液6ml,摇匀,加入
This article describes the bipyridine glyoxal mono (2-pyridyl) hydrazone (DMPH) reagent and vitamin complex preparations of cobalt color to produce a complex spectrophotometric determination of the method is simple, accurate and specific. Methods: Weigh 6 pieces of compound vitamin tablets (Hidropoliuit Mineral), ground into powder, accurately weighed amount of 3 tablets of fine powder to a 50ml beaker, add water 255ml and 60% perchloric acid 1.5ml, slowly heating, stirring, Let cool, filter to 50ml volumetric flask, add water to the mark.The precise amount of filtrate 2.5ml to 25ml volumetric flask, add 5% sodium citrate disodium solution 10ml, 12.5% (w / v) thiourea dimethyl For the formamide solution to dilute sodium hydroxide solution to adjust the pH to 4 to 7, plus 0.04% (w / v) DMPH ethanol solution 6ml, shake, add