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目的:观察体外培养的大鼠大脑皮质星形胶质细胞在模拟脑缺血再灌注损伤中的活性、存活率和无血清条件培养液蛋白含量的变化以及中药抗呆Ⅰ号的影响。方法:先对分离纯化培养的星形胶质细胞进行体外模拟脑缺血再灌注损伤模型的建立,然后采用MTT法对星形胶质细胞的活性和存活率进行测定以及用Bradform法对星形胶质细胞条件培养液中蛋白质含量进行测定。结果:体外培养的大鼠大脑皮质星形胶质细胞在体外模拟脑缺血再灌注损伤中的活性、存活率、无血清条件培养液蛋白含量的变化具有一定的规律性,可分细胞损伤期、功能代偿期、功能低下期和功能恢复期。抗呆Ⅰ号可使受损的星形胶质细胞的活性明显增强(与模型组相比多数时间点比较(t=2.074,P<0.05)。存活率显著提高(与模型组相比多数时间点P<0.05,t>2.219),使其条件培养液中的蛋白含量迅速增高(与模型组相比多数时间点,t>5.087,P<0.001)。结论:抗呆Ⅰ号可能通过保护星形胶质细胞免受损伤或使其分泌功能增强,从而间接地发挥星形胶质细胞对神经元的保护和修复作用。
OBJECTIVE: To observe the activity, survival rate and protein content of serum-free medium in simulated cerebral ischemic reperfusion injury in rat cerebral cortical astrocytes cultured in vitro and the effect of traditional Chinese medicine Anti-deaf I. METHODS: The isolated and purified cultured astrocytes were first established in vitro and simulated by cerebral ischemia reperfusion injury. The activity and survival rate of astrocytes were measured by MTT assay and the star was analyzed by Bradform method. The glial cell conditioned medium was assayed for protein content. RESULTS: The activity, survival rate and protein content of serum-free medium in rat brain cortical astrocytes cultured in vitro have certain regularity in simulating cerebral ischemia-reperfusion injury. , functional compensation period, low function period and functional recovery period. Anti-dead I markedly enhanced the activity of damaged astrocytes (compared with the model group at most time points (t=2.074, P<0.05). The survival rate was significantly increased (as compared with the model group for most of the time. (P<0.05, t>2.219), which rapidly increased the protein content in the conditioned media (t>5.087, P<0.001 at most time points compared with the model group).Conclusion: Kangda I may pass through the protection star. The glial cells are protected from injury or enhance their secretory function, which indirectly exerts the protection and repair of neurons by astrocytes.