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作者比较了三组不同临床特征的丝虫病人在接受海群生(DEC)长期治疗前后对丝虫抗原的细胞免疫应答。研究对象选自印度尼西亚的马来丝虫流行区,经寄生虫学和临床检查后分为微丝蚴血症组(17例);无症状无微丝蚴血症组(13例);象皮肿病人组(13例)。全部病例用DEC(100mg/wk)治疗一年。治疗前后采集每个病人的血清,用Ficoll-Isopaque密度梯度离心法分离外周血单核细胞(PBMC),置液氮中冷冻保存。应用ELISA测定血清中抗丝虫IgG_4含量。观察淋巴细胞
We compared the cellular immune responses to filarial antigens in three groups of filariasis patients with different clinical characteristics before and after long-term treatment with DEC (DEC). The subjects were selected from the endemic area of Malayan filariasis in Indonesia and divided into microfilamentosis group (17 cases) by parasitology and clinical examination; asymptomatic microfilariaemia group (13 cases); elephant skin Swollen patients (13 cases). All cases were treated with DEC (100 mg / wk) for one year. Serum from each patient was collected before and after treatment. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Isopaque density gradient centrifugation and stored in liquid nitrogen for cryopreservation. Serum anti-filarial IgG4 content was determined by ELISA. Observe lymphocytes