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目的探讨白藜芦醇对大鼠重症急性胰腺炎肺损伤时NF-κB活化的影响,以研究其对急性胰腺炎肺损伤的治疗作用及作用机制。方法 30只大鼠随机分为3组(n=10):假手术组(SO)、重症急性胰腺炎模型组(SAP)、白藜芦醇治疗组(RES)。5%牛黄胆酸钠逆行胰胆管穿刺注射复制大鼠重症急性胰腺炎模型,尾静脉注射10g/L白藜芦醇悬混液(0.1ml/100g),6h后处死大鼠,观测腹水量、血清淀粉酶、肺湿/干重比和肺组织病理学评分及NF-κB活性。结果 SAP组腹水量、血清淀粉酶、肺湿/干比重、病理学评分、NF-κB阳性率明显高于假手术组(P<0.05),RES组上述各项指标高于假手术组,但明显低于SAP组(P<0.05)。结论白藜芦醇能减轻大鼠重症急性胰腺炎造成的肺损伤,对其有一定的治疗作用,其作用机制可能与抑制NF-κB的表达有关。
Objective To investigate the effect of resveratrol on activation of NF-κB in lung injury of severe acute pancreatitis in rats and its therapeutic effect on acute lung injury induced by acute pancreatitis and its mechanism. Methods Thirty rats were randomly divided into three groups (n = 10): sham operation group (SO), severe acute pancreatitis model group (SAP) and resveratrol treatment group (RES). 5% sodium taurocholate transcatheter cholangiopancreatography injection of rat model of severe acute pancreatitis, tail vein injection of 10g / L resveratrol suspension (0.1ml / 100g), 6h after the rats were sacrificed to observe the amount of ascites, serum Amylase, lung wet / dry weight ratio and lung histopathological score and NF-κB activity. Results The ascites, serum amylase, lung wet / dry weight, pathological score and NF-κB positive rate in SAP group were significantly higher than those in sham operation group (P <0.05). The above indexes in SAP group were higher than those in sham operation group Significantly lower than SAP group (P <0.05). Conclusion Resveratrol can attenuate lung injury induced by severe acute pancreatitis in rats and may have a therapeutic effect on it. Its mechanism may be related to the inhibition of NF-κB expression.