Efficacy of Silene arenosa extract on acetylcholinesterase in Bungarus sindanus(krait)venom

• 作 者: Adil Shah Mushtaq Ahmed Naila Sher Nadia Mushtaq Rahmat Ali Khan Fozia Midrarullah
• 机 构: Department of Biotechnology,Faculty of Biological Sciences,University of Science&Technology,Bannu 28
• 出 处: 中医杂志（英文版）
• 发表日期: 2021年3期
• 关键词: acetylcholinesterase snake venoms si-lene arenosa competitive inhibition

OBJECTIVE:To examine the efficacy of Silene arenosa extract on acetylcholinesterase(AChE)of krait(Bungarus Sindanus)snake venom.METHODS:The present project designed to evalu-ate the inhibition of AChE by following standard procedures.RESULTS:Statistical analysis of the results showed that Silene arenosa exerted 73％inhibition against the krait venom acetylcholinesterase at fixed sub-strate acetylcholine(ACh)concentration(0.5 mM).Kinetic analysis using the Lineweaver Burk plot re-vealed that Silene arenosa caused a competitive type of inhibition i.e.Km values increased from 26.6 to 93.3 mM(26.6％to 93.3％)and Vmax remained con-stant in a concentration-dependent manner.Silene arenosa competes with the substrate to bind at the active site of the enzyme.The Kmapp of venom AChE for Silene arenosa increased from 60％to 81.6％and the Vmaxapp remains constant.Ki(inhibition con-stant was estimated to be 48 μg for snake venom;while the Km(Michaelis-Menten constant of AChE-substrate into AChE and product)was estimated to be 0.5 mM.The IC50 of AchE calculated for Silene arenosa was 67 μg.CONCLUSION:The present results suggest that Si-lene arenosa extract can be considered as an inhibi-tor of snake venom AChE.