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目的:探讨游离脂肪酸(free fatty acids,FFAs)异常对人肾癌786-O细胞增殖以及整合素连接激酶(inte-grin-linked kinase,ILK)蛋白表达的影响。方法:分别用0、0.05、0.1和0.2 mmol/L的油酸(oleic acid,OA)作用人肾癌786-O细胞48 h,其中0 mmol/L组作为对照组。利用MTT方法检测各组细胞增殖情况,流式细胞仪检测各组细胞凋亡情况,Western blot方法检测各组细胞中ILK、Akt和p-Akt蛋白的表达水平。结果:MTT检测结果显示,0.05、0.1和0.2 mmol/L OA组与对照组相比,细胞增殖活性显著增高(光密度0.657±0.056、0.682±0.028、0.718±0.042 vs.0.495±0.034,P均<0.001);而不同浓度的OA对786-O细胞的凋亡作用并不明显(凋亡率2.42%±0.25%vs.2.33%±0.87%vs.2.25%±0.51%,P=0.082);与对照组相比,OA组中的ILK、p-Akt蛋白表达显著上调。结论:FFAs通过刺激ILK/Akt通路可促进细胞增殖,可能与肾癌发生有关。
OBJECTIVE: To investigate the effects of abnormalities of free fatty acids (FFAs) on the proliferation of human renal carcinoma 786-O cells and the expression of intestine-linked kinase (ILK). Methods: Human renal carcinoma 786-O cells were treated with 0, 0.05, 0.1 and 0.2 mmol / L oleic acid (OA) for 48 h, respectively, and 0 mmol / L as control group. The proliferation of each group was detected by MTT method. The apoptosis of each group was detected by flow cytometry. The protein expression of ILK, Akt and p-Akt in each group was detected by Western blot. Results: The results of MTT assay showed that the cell proliferation activity was significantly increased in the groups of 0.05, 0.1 and 0.2 mmol / L OA compared with the control group (optical density 0.657 ± 0.056,0.682 ± 0.028,0.718 ± 0.042 vs.0.495 ± 0.034, P <0.001). However, the apoptosis of 786-O cells was not obvious at different concentrations of OA (apoptosis rate 2.42% ± 0.25% vs.2.33% ± 0.87% vs.2.25% ± 0.51%, P = 0.082); Compared with the control group, OA group OAK, p-Akt protein expression was significantly increased. Conclusion: FFAs can promote cell proliferation by stimulating ILK / Akt pathway, which may be related to the occurrence of renal cell carcinoma.