Secretory expression and characterization of a recombinantdeleted variant of human hepatocyte growth

• 作 者: Zhi-Min Liu Hong-Liang Zhao Ch
• 机 构: DepartmentofMicrobiologicalEngineering
• 出 处: 世界胃肠病学杂志：英文版（电子版）
• 发表日期: 2005年45期
• 关键词: 分泌腺 基因表达 肝细胞生长因子 人体细胞 Hepatocyte growth factor Pichia pastoris Secretory expres
• 基金项目: 国家高技术研究发展计划(863计划)，the Ministry of Science and Technology of China

AIM: To study the secretory expression of human hepatocyte growth factor (hdHGF) gene in Pichia pastoris.METHODS: The full-length gene of human cDNA encoding the deleted variant of hdHGF was cloned by RT-PCR and overlapping-fragment PCR technique using mR