论文部分内容阅读
肌梭是一种本体感受器,广泛分布在全身的肌肉中,在四肢肌肉中特别多,我们在进行肌肉组织染色比较时,经过实践,发现该方法简单可行,特介绍如下。1.取材:哺乳动物的四肢肌肉(近腱处)。2.固定:取新鲜材料3毫米左右,人固定液(水合氯醛5克,无水酒精25毫升,蒸馏水25毫升)固定24小时。3.方法:(1)取出材料,用蒸馏水冲洗,入氨酒精(无水酒精100毫升,氧氢化氨0.5毫升)中24小时。(2)去除氨酒精,流水冲洗12-24小时,用蒸馏水洗多次,直至洗去组织上多余的氨酒精。(3)入2%硝酸银水溶液一周(37℃温箱)。
Muscle spindle is an ontological sensor, widely distributed in the body muscles, particularly in the limbs muscles, we compared muscle tissue staining, through practice, found that the method is simple and feasible, introduced as follows. 1. Drawn: Mammals limbs muscles (near tendon Office). 2. Fixed: Take about 3 millimeters of fresh material, fixed liquid (chloral hydrate 5 grams, 25 ml of anhydrous alcohol, distilled water 25 ml) for 24 hours. 3. Methods: (1) Remove the material, rinse with distilled water, into the ammonia alcohol (anhydrous ethanol 100 ml, hydrogen peroxide 0.5 ml) for 24 hours. (2) to remove ammonia alcohol, running water for 12-24 hours, washed with distilled water several times until the wash away excess ammonia alcohol. (3) into 2% silver nitrate aqueous solution for one week (37 ℃ incubator).