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目的探讨贾第鞭毛虫和隐孢子虫染色方法异硫氰酸荧光素(FITC)-滤膜法,并将其与现行的国家标准方法FITC法进行比较。方法用FITC-滤膜法和FITC法分别染色贾第鞭毛虫和隐孢子虫标准体,对其染色所需时间、染色效果、检出率等逐一进行对比。结果 FITC-滤膜法的样品固定时间为5 min,远远少于FITC法的60 min,FITC-滤膜法的综合染色时间为42 min,也远少于FITC法的97 min。FITC-滤膜法对贾第鞭毛虫和隐孢子虫的检出率分别为(96.33±1.08)%和(96.67±1.70)%,高于FITC法的(94.00±2.16)%和(92.67±2.05)%,FITC-滤膜法染色可清晰看到贾第鞭毛虫和隐孢子虫的细胞核,优于FITC法。结论 FITC-滤膜法缩短了染色时间,且便于之后的分子生物学分析,可作为贾第鞭毛虫和隐孢子虫染色的替代方法使用。
OBJECTIVE To investigate the staining method of Giardia lamblia and Cryptosporidium parvum by FITC-membrane method and to compare with the current national standard FITC method. Methods The standard of Giardia lamblia and Cryptosporidium parvum were stained with FITC-FITC and FITC respectively. The time required for staining, the staining effect and the detection rate were compared one by one. Results The FITC-filter method had a fixed time of 5 min, which was far less than that of the FITC method for 60 min. The FITC-filter method had a total staining time of 42 min and was far less than the 97 min of the FITC method. The detection rates of FITC-filter on Giardia and Cryptosporidium were (96.33 ± 1.08)% and (96.67 ± 1.70)%, respectively, higher than those of FITC method (94.00 ± 2.16)% and (92.67 ± 2.05) )%, FITC-membrane staining can clearly see the nuclei of Giardia and Cryptosporidium, better than the FITC method. Conclusion The FITC-filter method can shorten the staining time and facilitate subsequent molecular biology analysis. It can be used as an alternative method for staining Giardian and Cryptosporidium.