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In this paper,the concept of reversed-or normal-phase chiral stationary phase liquidchromatography has been put forward according to the polar strength of mobile and stationary phases.The statistical model developed in HPLC has been used to investigate the separation mechanism ofD-and L-enantiomer in chiral stationary phase liquid chromatography.It has been observed thatthe variation of capacity factor of enantiomers with mobile phase composition in both reversed-phaseand normal-phase chiral stationary phase liquid chromatography can be described by the fundamentalelution equation lnk’=a+blnC_b+cC_b.The effect of mobile phase composition on the selec-tivity of enantiomers D and L in normal-phase chiral stationary phase liquid chromatography cam bedescribed by the equation lnα=⊿a+⊿blnC_b,but in reversed-phase chiral stationary phaseliquid chromatography the selectivity is almost independant of the mobile phase composition.
In this paper, the concept of reversed-phase normal-phase chiral stationary phase liquid chromatography has been put forward according to the polar strength of mobile and stationary phases. The statistical model developed in HPLC has been used to investigate the separation mechanism of D- and L -enantiomer in chiral stationary phase liquid chromatography. It has been observed that the variation of capacity factor of enantiomers with mobile phase composition in both reversed phase-normal-phase chiral stationary phase liquid chromatography can be described by the fundamentale equation lnk ’= a + blnC_b + cC_b. The effect of mobile phase composition on the selec-tivity of enantiomers D and L in normal-phase chiral stationary phase liquid chromatography cam bedescribed by the equation lnα = ⊿a + ⊿blnC_b, but in reversed-phase chiral stationary phase liquid chromatography the selectivity is almost independant of the mobile phase composition.