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籼稻品种Dular在低温条件下幼苗会表现失绿.前期遗传研究表明,这种低温失绿的表型是由一个隐性基因控制的,该基因初步定位在第9染色体长臂的SSR标记RM257和RM242之间,将该基因暂命名为cisc(t).本研究在前期初步定位的基础上,利用粳稻品种Lemont(低温条件下幼苗表现正常绿色)与Dular杂交,构建了一个很大的F2群体,将cisc(t)基因精细定位在一个12kb的区间内.该区间只含有一个注释基因,编码一个三角状五肽重复区(PPR)蛋白.测序结果表明,Dular在该基因的第60位碱基处缺失了8个碱基,造成移码突变而失去正常功能.这与Dular表现白化突变表型相吻合.而且,前人研究表明,许多植物的幼苗失绿突变性状与PPR蛋白有关.因此,将该基因确定为cisc(t)的候选基因.
Indica rice seedling Dular showed chlorosis at low temperature.Previous genetic studies showed that this low temperature chlorosis phenotype was controlled by a recessive gene that was initially located on the long arm of chromosome 9, SSR markers RM257 and RM242, the gene was tentatively named as cisc (t) .In this study, a large F2 population was constructed based on the preliminary location of the early stage using the Lemont (normal green seedlings under low temperature conditions) hybridization with Dular , The cisc (t) gene was finely mapped in a 12kb interval, containing only one annotation gene encoding a triangular pentapeptide repeat region (PPR) protein.The sequencing results showed that Dular gene at position 60 Base deletion of 8 bases, resulting in frameshift mutations and loss of normal function.This is consistent with the appearance of Dular phenotype albinism mutation.Moreover, previous studies have shown that many plant seedling chlorotic mutation traits associated with the PPR protein.Therefore, This gene was identified as a candidate gene for cisc (t).