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目的∶建立检测弓形虫 Ｉｇ Ｍ 抗体的免疫吸附凝集试验 （ Ｉ Ｓ Ａ Ｇ Ａ） 。方法∶ Ｕ 型微孔板以适宜浓度的羊抗人 Ｉｇ Ｍ 抗体包被， 用１ ％ 牛血清白蛋白封闭， 洗板后加入待测血清， 在３７ ℃孵育后洗涤， 加入弓形虫（ Ｒ Ｈ 株） 速殖子抗原悬液， 置３７ ℃过夜后观察结果。将其与丹麦的 Ｉ Ｓ Ａ Ｇ Ａ 和 Ｅ Ｌ Ｉ Ｓ Ａ 检测的结果以及玻片 Ｅ Ｉ Ａ 检测的结果作比较。结果∶本法与丹麦的试验检测丹麦孕妇４４ 份血清， 总符合率为９３２ ％ ；与玻片 Ｅ Ｉ Ａ 检测丹麦和上海孕妇６７ 份血清， 总符合率为９２５ ％ ， 两法的滴度之间明显相关 （γ＝ ０５８９ ， Ｐ＜ ０００１） ， Ｉ Ｓ Ａ Ｇ Ａ 滴度较玻片 Ｅ Ｉ Ａ 高１８ 倍； 用 Ｗ Ｈ Ｏ 国际生物标准化实验室提供的标准的抗弓形虫人血清定量测得其灵敏度为００８ Ｉ Ｕ／ ｍｌ。结论∶ Ｉｇ Ｍ Ｉ Ｓ Ａ Ｇ Ａ 具有敏感性、特异性高， 操作简便等优点， 不仅可作为弓形虫急性感染和慢性感染活动期的一种检测手段， 而且适用于弓形虫感染调查的大规模筛选。
Objective: To establish an immunoadsorbent agglutination test (IgA) for the detection of IgM antibodies to Toxoplasma gondii. Methods: U-plate was coated with goat anti-human Ig M antibody at a suitable concentration and blocked with 1% bovine serum albumin. The plates were washed with the serum to be tested, incubated at 37 ° C and washed. Toxoplasma gondii Strain) tachyzoite antigen suspension, set at 37 ° C overnight after the observation. This was compared with the results of I S A G A and E L I S A tests in Denmark and the results of slide E I A tests. Results: This method and Danish test detected 44 serums of pregnant women in Denmark, with a total coincidence rate of 932%. Serum samples from 67 pregnant women in Denmark and Shanghai were detected with slide EⅠA. The total coincidence rate was 925% (Γ = 0589, P <0001). The titer of I S A G A was 18 times higher than that of slide E I A. The standard provided by WHO International Bio-standardization Laboratory The anti-Toxoplasma gondii serum was measured quantitatively with a sensitivity of 0. 08 I U / ml. Conclusion: Ig MIASA G is sensitive, specific and easy to operate. It not only can be used as a detection method for Toxoplasma gondii acute infection and chronic infection, but also suitable for the survey of Toxoplasma gondii infection Size screening.