在酵母中乙酰辅酶A产生酶

• 出 处: 乙醛醋酸化工
• 发表日期: 2014年09期
• 关键词: 酮酸酯 核苷酸序列 醋酸酯 乙醛 杂多 突变细胞 酵母 乙醛脱氢酶 表达载体 合成酶

本发明涉及到鉴别具有酶活性的杂多肽的方法,杂多肽用于转化酵母细胞中的丙酮酸酯,乙醛或醋酸酯成乙酰辅酶A,方法包括:1)提供包含缺失(PDH)旁-路的至少一个基因,基因从编码酶丙酮酸酯脱羧酶(PDC),乙醛脱氢酶(ALD)和乙酰辅酶A合成酶(ACS)中选择;2)转化所说具有表达载体的突变细胞,其包括杂核苷酸序列,核苷酸序列编码候选多肽,其具有转化丙酮酸酯,乙醛或醋酸酯为乙酰辅酶A的潜在的酶活性;

The present invention relates to a method of identifying a heteropeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate to yeast acetyl-CoA in yeast cells, comprising: 1) providing a polypeptide comprising a deletion (PDH) Pathway, genes are selected from enzymes encoding pyruvate decarboxylase (PDC), aldehyde dehydrogenase (ALD) and acetyl-CoA synthetase (ACS); 2) transforming said mutant cells with an expression vector , Which comprises a heterologous nucleotide sequence encoding a candidate polypeptide having the potential enzymatic activity of converting pyruvate, acetaldehyde or acetate to acetyl-CoA;