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为探明3,4,5-三羟基苯甲酸甲酯(MG)抑制茄青枯拉尔氏菌的机制,利用二维电泳技术,分析了MG作用下茄青枯拉尔氏菌Rs-T02菌株的差异蛋白。结果发现在MG作用下的菌体中检测出29个差异蛋白点。采用质谱技术鉴定出22个差异蛋白,其中新增蛋白11种、缺失蛋白5种、表达上调蛋白1种和表达下降蛋白5种。这些蛋白参与能量代谢、信号转导、物质运输和DNA修复等途径。我们推测,与能量代谢相关的新增蛋白spot 9和缺失蛋白spot 65可能与MG抑制茄青枯拉尔氏菌的机制有关。
In order to investigate the mechanism of inhibition of B. solanacearum by 3,4,5-trihydroxybenzoate (MG), two-dimensional electrophoresis was used to analyze the effects of MG on the growth of R. solanacearum Rs-T02 Strain differential proteins. The results showed that in the role of MG in the bacteria detected 29 differential protein spots. Twenty-two differential proteins were identified by mass spectrometry, including 11 newly-added proteins, 5 absent proteins, 1 up-regulated protein and 5 down-regulated proteins. These proteins are involved in energy metabolism, signal transduction, material transport and DNA repair and other ways. We speculate that the additional protein spot 9 and the missing protein spot 65 related to energy metabolism may be related to the mechanism by which MG inhibits B. solani.