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目的:观察抗凋亡蛋白Survivin和Ki-67在原发性肝癌(PHC)中的表达并探讨其临床意义。方法:采用免疫组织化学S-P法检测34例原发性肝癌组织、14例癌旁正常肝组织中survivin和Ki-67的表达情况。结果:原发性肝癌组织中survivin的阳性表达定位于细胞浆和细胞核,阳性表达率明显高于癌旁正常肝组织(P<0.05);Ki-67的阳性表达主要定位于细胞核,其阳性表达率亦明显高于癌旁正常肝组织(P<0.05)。二者的阳性表达与原发性肝癌患者的性别、年龄、肿瘤大小无相关性(P>0.05),与淋巴结转移、组织分化程度及癌栓是否形成有关(P<0.05);此外,二者在原发性肝癌组织中的表达呈显著正相关(P<0.05)。结论:Survivin和Ki-67在原发性肝癌组织中表达上调,均与原发性肝癌的组织分化程度及癌栓是否形成密切相关;检测survivin和Ki-67的表达有助于原发性肝癌的预防、治疗和预后评估。
Objective: To observe the expression of anti-apoptotic proteins Survivin and Ki-67 in primary hepatocellular carcinoma (PHC) and to investigate their clinical significance. Methods: The expressions of survivin and Ki-67 in 34 cases of primary hepatocellular carcinoma and 14 cases of adjacent normal liver tissues were detected by immunohistochemical S-P method. Results: The positive expression of survivin in primary hepatocellular carcinoma was localized in the cytoplasm and nucleus, and the positive expression rate was significantly higher than that in the adjacent normal liver tissue (P <0.05). The positive expression of Ki-67 mainly localized in the nucleus and was positive The rate was also significantly higher than normal liver adjacent tissues (P <0.05). There was no correlation between the positive expression of these two factors and the gender, age and tumor size of patients with primary liver cancer (P> 0.05), but also with lymph node metastasis, histological differentiation and the formation of tumor thrombus (P <0.05). In addition, There was a significant positive correlation between the expression of HCC and primary liver cancer (P <0.05). Conclusion: The upregulation of Survivin and Ki-67 in primary hepatocellular carcinoma is closely related to the histological differentiation of primary hepatocellular carcinoma and the formation of tumor suppository. The detection of survivin and Ki-67 expression is helpful for the diagnosis of primary hepatocellular carcinoma Prevention, treatment and prognosis assessment.