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目的 研究TNF-α对胶质瘤细胞的抑制作用及其对Bcl-2蛋白表达的影响。方法 应用细胞计数法和MTT法研究经TNF-α处理的大鼠C6胶质瘤细胞的生长和增殖活性,研究了细胞的形态学变化以及用免疫细胞化学方法检测PCNA(增殖细胞核抗原)和Bcl-2蛋白的表达。结果 TNF-α对体外培养C6胶质瘤细胞生长和增殖具有呈时间和剂量依赖性的抑制作用,下调PCNA的表达,并诱导细胞凋亡,但对抑凋亡蛋白Bcl-2的表达无明显影响。结论 TNF-α对恶性胶质瘤细胞具有抑制生长和诱导凋亡作用,对Bcl-2蛋白表达没有影响。
Objective To study the inhibitory effect of TNF-α on glioma cells and its effect on Bcl-2 protein expression. Methods The cell growth and proliferation of rat C6 glioma cells treated with TNF-α were studied by cell counting and MTT assay. The morphological changes of cells and the expression of PCNA (proliferating cell nuclear antigen) and Bcl-2 -2 protein expression. Results TNF-α inhibited the growth and proliferation of C6 glioma cells in vitro in a time-and dose-dependent manner, down-regulated the expression of PCNA and induced the apoptosis, but had no obvious effect on the expression of Bcl-2 influences. Conclusion TNF-α can inhibit the growth and induce the apoptosis of malignant glioma cells, but has no effect on the expression of Bcl-2 protein.