论文部分内容阅读
目的:建立奥扎格雷纳米结构脂质载体(ozagrel-loaded nanostructured lipid carriers,OZ-NLC)包封率的测定方法。方法:分别采用超滤法、葡聚糖凝胶柱层析法、超速离心法分离游离药物,以紫外分光光度法测定奥扎格雷的含量,计算包封率。结果:超滤法和葡聚糖凝胶柱层析法可有效分离OZ-NLC与游离药物,两种方法的平均回收率分别为98.31%和97.15%,平均包封率为59.21%和61.11%。结论:葡聚糖凝胶柱层析法操作简便,结果可靠,重现性好,故选择该方法测定OZ-NLC的包封率。
Objective: To establish a method for determination of encapsulation efficiency of ozagrel-loaded nanostructured lipid carriers (OZ-NLC). Methods: The free drugs were separated by ultrafiltration, Sephadex LH-20 and ultracentrifugation respectively. The content of ozagrel was determined by ultraviolet spectrophotometry. The entrapment efficiency was calculated. Results: The results showed that the average recoveries of OZ-NLC and free drug were 98.31% and 97.15%, respectively. The average entrapment efficiencies were 59.21% and 61.11% . Conclusion: Dextran gel column chromatography is simple, reliable and reproducible. Therefore, this method was used to determine the entrapment efficiency of OZ-NLC.