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目的:研究调亡相关基因Fas/FasL在正常口腔黏膜、上皮异常增生和口腔鳞癌组织中的表达及意义。方法:应用免疫组织化学方法检测10例正常口腔黏膜、26例上皮异常增生、38例口腔鳞癌组织及肿瘤浸润淋巴细胞(TIL)中Fas/FasL的表达。结果:Fas在正常口腔黏膜中广泛表达;上皮异常增生和鳞癌组织表达明显下调(P<0.05);Fas表达与口腔鳞癌分化程度有关;FasL在正常口腔黏膜不表达;上皮异常增生和鳞癌组织表达明显上调(P<0.05);FasL表达与口腔鳞癌分化程度无关(P>0.05);TIL细胞Fas、FasL阳性表达率为81.6%和84.2%。结论:Fas表达与口腔黏膜上皮细胞的自然分化成熟、衰老及口腔鳞癌的形成和肿瘤的恶性度有关;FasL的表达上调可能是口腔鳞癌组织免疫反攻击的体现;Fas、FasL可作为监测口腔上皮癌变的标记物。
OBJECTIVE: To study the expression and significance of apoptosis-related gene Fas / FasL in normal oral mucosa, epithelial dysplasia and oral squamous cell carcinoma. Methods: The expressions of Fas / FasL in 10 cases of normal oral mucosa, 26 cases of epithelial dysplasia, 38 cases of oral squamous cell carcinoma and tumor infiltrating lymphocytes (TIL) were detected by immunohistochemistry. Results: Fas was widely expressed in normal oral mucosa; epithelial dysplasia and squamous cell carcinoma tissues were significantly down-regulated (P <0.05); Fas expression was correlated with the differentiation of oral squamous cell carcinoma; FasL was not expressed in normal oral mucosa; epithelial dysplasia and squamous cell carcinoma (P <0.05). The expression of FasL was not related to the differentiation of oral squamous cell carcinoma (P> 0.05). The positive rates of Fas and FasL in TIL were 81.6% and 84.2%, respectively. Conclusion: Fas expression is related to the natural maturation of oral mucosal epithelial cells, aging and the formation of oral squamous cell carcinoma and the malignancy of the tumor. The upregulation of FasL may be the manifestation of the immune counterattack in oral squamous cell carcinoma. Fas and FasL may be used as monitoring Marker of oral carcinogenesis.