论文部分内容阅读
目的 :查明西藏林芝地区是否存在肾综合征出血热 (HFRS)自然疫源地。方法 :选择有疫情报告地区 ,进行人间疫情调查 ;健康人群特异性抗体检测 ;在山地针、阔叶混合林及山间平原的农田和居民区 ,用夹夜法调查小兽数量 ;采集捕获动物肺、血和牛、羊血清标本 ,检测汉坦病毒感染情况。血标本用金标免疫斑点渗滤试验和间接免疫荧光法检测抗体 ;动物肺的冷冻切片用免疫荧光法检测汉坦病毒抗原。结果 :在西藏自治区卫生防疫站疫情资料中 ,有该病记载的 1976年工布江达县 1例 ,1980年林芝县 9例 ;检测林芝地区医院住院者 18人、西藏高考学生 35 7人和其他体检人员 117人血标本 ,仅从 2名林芝县、2名工布江达县、1名察隅县的高考学生和 1名住院产妇中检出抗汉坦病毒总抗体和IgG荧光抗体 ,被检人群隐性感染率为 1.2 2 %。检测中华姬鼠等脊椎动物肺 115只 ,汉坦病毒抗原阳性的中华姬鼠 1只 ,带病毒率 0 .87% ;动物血抗汉坦病毒总抗体和IgG荧光抗体皆阳性的有中华姬鼠 5只、高山田鼠 1只 ,抗体阳性率为 5 .2 2 % ;检测牛、羊 310只血清 ,其中黄牛抗汉坦病毒总抗体阳性率0 .85 % ( 1/ 118) ,绵羊总抗体和IgG抗体阳性率1.6 8% ( 3/ 179)。结论 :西藏林芝地区有HFRS散发病例 ,健康人群、中华姬鼠、高山田鼠、黄牛和绵羊?
Objective: To find out whether there is a natural epidemic of hemorrhagic fever with renal syndrome (HFRS) in Linzhi area of Tibet. Methods: The epidemic situation was selected for the investigation of human epidemic situation; the detection of specific antibodies in healthy population; the investigation of the number of small animals in the fields and residential areas of mountain acreage, broadleaf mixed forest and mountain plain; Lung, blood and cattle, sheep serum samples, detection of Hantavirus infection. Antibodies were detected by gold standard immunofluorescence stain and indirect immunofluorescence in blood samples; Hantavirus antigen was detected by immunofluorescence in frozen section of animal lung. Results: Among the epidemic data of the epidemic prevention and control stations in the Tibet Autonomous Region, there were 1 case reported in 1976 in Gongbu County and 9 cases in Linzhi County in 1980; 18 cases were hospitalized in Nyingchi District; 357 were students from Tibet National Entrance Examination; and other medical staff 117 human blood samples were detected only from two Nyingchi County, two workers cloth Jiangda County, a Zayu County entrance exam students and a hospitalized anti-Hantaan virus antibodies and IgG fluorescent antibodies were seized in the crowd of latent infection The rate was 1.2 2%. A total of 115 vertebrate lungs, including Apodemus agrarius, were detected. One Hamster mouse with positive Hantavirus antigen had a viral load of 0.87%. Animals with anti-Hantavirus total antibody and IgG fluorescent antibody were positive with Apodemus aganum 5 mice and 1 highland vole. The positive rate of antibody was 5.22%. The serum of 310 cattle and sheep was detected. The positive rate of total anti-Hantaan virus in cattle was 0.85% (1/118) IgG antibody positive rate of 1.6 8% (3/179). Conclusion: There are HFRS cases, healthy people, Apodemus agrarius, alpine vole, cattle and sheep in Linzhi area, Tibet.