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为探讨甘露寡糖(MOS)对异育银鲫生长、免疫、肠道组织结构及抗病力的影响,试验选取360尾异育银鲫[初均重(16.19±0.03)g],随机分成5组、每组3个重复,在日粮中添加不同浓度甘露寡糖(0、60、120、240、480 mg/kg),连续投喂80d,并于第80天时进行嗜水气单胞菌感染,测定异育银鲫生长、免疫、肠道结构等指标及异育银鲫抗嗜水气单胞菌感染的能力。试验结果表明,无论投喂50d,还是80d,甘露寡糖对鱼体的生长指标(特定生长率、增重率、蛋白质效率、饵料系数)均没有显著影响(P>0.05);投喂50d,与对照组比,甘露寡糖能显著提高血清球蛋白浓度(P<0.05);投喂80d后,与对照组比,240、480 mg/kg甘露寡糖组能显著提高碱性磷酸酶活性,甘露寡糖组能显著提高血清球蛋白浓度(P<0.05),480 mg/kg甘露寡糖组能显著提高血清总抗氧化能力,120、240 mg/kg甘露寡糖组能显著提高肠褶皱襞长(P<0.05),对皱襞间质宽、黏膜下层宽没有显著影响(P>0.05),肌层宽随着MOS的添加有增加趋势(P>0.05);嗜水气单胞菌感染后,与对照组比,240、480 mg/kg甘露寡糖组成活率提高了22.6%,免疫保护率达45.4%。日粮中添加甘露寡糖组对鱼体肝脏HSP70基因表达没有显著影响(P>0.05)。因此,添加240、480 mg/kg甘露寡糖能提高鱼体的免疫能力,增强鱼体抗病原菌感染能力。
To investigate the effect of MOS on the growth, immunity, gut tissue structure and disease resistance of allogynogenetic crucian carp (Carassius auratus gibelio), 360 heterophylla crucian carp (average initial weight was (16.19 ± 0.03) g] were randomly divided into 5 groups with 3 replicates in each group. Different concentrations of mannooligosaccharides (0, 60, 120, 240 and 480 mg / kg) were added into the diets and fed continuously for 80 days. On the 80th day, Infection, determination of allogynogenetic growth, immune, intestinal structure and other indicators and allogynogenetic crucian carp Aeromonas hydrophila infection ability. The results showed that mannooligosaccharides had no significant effect on the growth index (specific growth rate, weight gain rate, protein efficiency, feed conversion coefficient) of the fish no matter fed 50 d or 80 d (P> 0.05) Compared with the control group, mannose oligosaccharide could significantly increase the concentration of serum globulin (P <0.05). After 80 days of feeding, compared with the control group, 240,480 mg / kg mannose oligosaccharide group could significantly increase alkaline phosphatase activity, Mannan oligosaccharide group could significantly increase serum globulin concentration (P <0.05), 480mg / kg mannose oligosaccharide group can significantly increase the total serum antioxidant capacity, 120,240 mg / kg mannose oligosaccharide group can significantly improve intestinal fold 襞(P> 0.05), and had no significant effect on the width of the interspace and the width of the submucosa (P> 0.05), and the width of the muscle layer increased with the addition of MOS (P> 0.05). After the infection of Aeromonas hydrophila, Compared with the control group, the viability of 240,480 mg / kg mannooligosaccharides increased by 22.6% and the immunoprotection rate reached 45.4%. The addition of mannooligosaccharides to diets had no significant effect on HSP70 gene expression in liver (P> 0.05). Therefore, adding 240,480 mg / kg mannooligosaccharides can improve the immune capacity of fish and enhance the ability of fish to resist pathogen infection.