论文部分内容阅读
目的:观察血管成形术后平滑肌细胞增生及再狭窄形成过程,探讨低分子量肝素的抑制作用.方法:以PT-CA导管对家兔髂动脉行血管成形术,于术后不同时间取血管成形段血管,行HE染色及增殖细胞核抗原(PCNA)兔疫组化染色,测量内膜/中膜比值,计数平滑肌细胞的增殖细胞核抗原阳性率.结果:血管成形术造成血管损伤,术后3天即见有少许平滑肌细胞迁移至内膜,至14天时内膜增厚,3、7、14天时中膜平滑肌细胞的增殖细胞核抗原阳性率分别为39.54%、10.78%、3.23%,低分子量肝素组不同时间内膜增厚程度及增殖细胞核抗原阳性率均低于血管成形术组.结论:血管成形术的损伤导致中膜平滑肌细胞增生并向内膜迁移,形成再狭窄病变,低分子量肝素能够部分抑制平滑肌细胞的增生及迁移,减少再狭窄形成.
OBJECTIVE: To observe the process of proliferation and restenosis of smooth muscle cells after vascular angioplasty and to investigate the inhibitory effect of low molecular weight heparin.Methods: The angioplasty of rabbit iliac artery was performed with PT-CA catheter and the angioplasty segment The blood vessels were stained with HE staining and proliferating cell nuclear antigen (PCNA), the ratio of intima / media was measured, and the positive rate of proliferating cell nuclear antigen (PCNA) in smooth muscle cells was counted.Results: Angioplasty caused vascular injury, A few smooth muscle cells migrated to the intima, and the thickening of the intima was observed on the 14th day. The positive rates of proliferating cell nuclear antigen (PCNA) were 39.54%, 10.78% and 3.23% at 3, 7 and 14 days respectively. The low molecular weight heparins The time of membrane thickening and the positive rate of proliferating cell nuclear antigen were lower than that of angioplasty group.Conclusion: The damage of angioplasty resulted in the proliferation of neointimal smooth muscle cells and their migration to the intima, forming the restenosis, and the low molecular weight heparin could partially inhibit Smooth muscle cell proliferation and migration, reduce the formation of restenosis.