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目的探讨玉郎伞水提物对大鼠离体心脏缺血再灌注损伤的作用及其机制。方法将大鼠随机分为5组,即正常对照组,模型组,玉郎伞水提物低、高剂量组(含生药量3,12 g·L-1),阳性对照药维拉帕米组(含维拉帕米5×10-7mol·L-1)。离体大鼠心脏采用Langendorff法灌流,停灌30 min再灌30 min造成心肌缺血-再灌注损伤模型。于左心室插入水囊导管,采用MPA心功能分析系统记录心脏血流动力学各项指标,测定冠脉流量(coronary artery flow,CF)和冠脉流出液中肌酸激酶(creatine kinase,CK)、肌酸激酶同工酶(MB isoenzyme of creatine kinas e,CK-MB)、乳酸脱氢酶(lactate dehydrogenase,LDH)、乳酸脱氢酶同工酶-1(lactate dehydrogenase isozyme-1,LDH-1)活性以及心肌组织超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量。结果与模型组比较,玉郎伞水提物高剂量组可明显改善缺血-再灌注损伤心功能的各项指标,降低CK、CK-MB、LDH、LDH-1的活性,增加心肌组织SOD的活性,减少MDA的产生。结论玉郎伞水提物对缺血-再灌注所致的心肌损伤具有保护作用,其作用机制可能与清除氧自由基、抑制脂质过氧化反应有关。
Objective To investigate the effect and its mechanism of aqueous extract of Ylang Lang on ischemia / reperfusion injury in isolated rat hearts. Methods The rats were randomly divided into 5 groups: normal control group, model group, low, high dose group of Ylangangluo water extract (containing crude drug amount 3,12 g · L-1), positive control drug verapamil Group (containing verapamil 5 × 10-7mol·L-1). The isolated rat heart was perfused with Langendorff method and the ischemia-reperfusion injury model was induced by 30 min reperfusion and 30 min reperfusion. The left ventricle was inserted into the balloon catheter, MPA cardiac function analysis system was used to record the indexes of cardiac hemodynamics. The coronary artery flow (CF) and creatine kinase (CK) , MB isoenzyme of creatine kinases (CK-MB), lactate dehydrogenase (LDH), lactate dehydrogenase isozyme-1 (LDH-1 ) Activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in myocardium were measured. Results Compared with the model group, the high dose Yulangsan water extract group could obviously improve the indexes of cardiac function, reduce the activity of CK, CK-MB, LDH and LDH-1 and increase the activity of SOD Activity, reduce the production of MDA. Conclusion The aqueous extract of Yulangsan has a protective effect on myocardial injury induced by ischemia - reperfusion. Its mechanism may be related to scavenging oxygen free radicals and inhibiting lipid peroxidation.