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研究干扰素 -脑啡肽融合蛋白的外周镇痛作用和机制。对小鼠进行热损伤诱导 ,采用经典热板法测定小鼠后肢脚趾外周涂抹干扰素、脑啡肽融合蛋白的痛阈变化 ,并用阿片选择性拮抗剂纳曲酮、纳络酮及干扰素单抗进行阻断试验。与干扰素母体相比 ,融合蛋白具有较强的外周镇痛作用 ,这种作用可被纳络酮、干扰素单抗逆转或阻断。融合蛋白具有较强镇痛功能 ,可作为外用镇痛候选药物 ,其作用机理与干扰素受体、阿片 μ受体有关
To investigate the peripheral analgesic effect and mechanism of interferon-enkephalin fusion protein. The mice were induced by thermal injury. The changes of pain threshold of interferon and enkephalin fusion protein in the hindlimb of the mouse hind limb were measured by the classical hot plate method. The opioid selective antagonists naltrexone, naloxone and interferon mono Anti-blocking test. Compared with the interferon matrix, the fusion protein has a strong peripheral analgesic effect, which can be reversed or blocked by naloxone and interferon monoclonal antibody. Fusion protein has strong analgesic function, can be used as topical analgesic drug candidate, its mechanism of action and interferon receptors, opiate mu receptor