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目的本研究旨在评定6-羟基多巴损伤纹状体后黑质和腹侧被盖区的变化,并探讨阿扑吗啡在该模型中可能存在的神经保护作用。方法6-羟基多巴损伤纹状体模型:在造模前15分钟,连续22天皮下注射阿扑吗啡10 mg/kg·d。在第5 周时,观察大鼠行为学(安啡他明诱导的旋转数目)、组织化学(TH/Nissl 染色后黑质和腹侧被盖区的多巴胺细胞)、神经化学(高压液相法测定纹状体的多巴胺含量)改变。结果阿扑吗啡不仅消弱安啡他明诱导的旋转数目,而且,显著减少黑质的损伤(与对照组相比,多巴胺细胞达到69%,细胞形态恢复正常)和腹侧被盖区的损伤(多巴胺细胞增加了60%,细胞形态恢复正常); 并且,阿扑吗啡显著消弱6-羟基多巴损伤纹状体的多巴胺含量,使DOPAC/DA率恢复正常。结论在6-羟基多巴损伤纹状体模型中,阿扑吗啡不仅有神经保护作用,而且有神经营养作用。但神经营养作用局限于腹侧被盖区,并随着用药时间延长而增加。
Objective This study was designed to evaluate the changes of substantia nigra and ventral tegmental area after 6-hydroxydopamine damage striatum and to explore the possible neuroprotective effects of apomorphine in this model. Methods 6-hydroxydopamine-induced striatum model: Apomorphine 10 mg / kg · d was administered subcutaneously for 22 consecutive days at 15 minutes before modeling. At week 5, rats were observed for behavioral (number of revolutions induced by bupivacaine), histochemistry (dopamine cells in the substantia nigra and ventral tegmental area after TH / Nissl staining), neurochemical (high pressure liquid phase method Determination of striatum dopamine content) changes. Results Apomorphine not only impaired the number of rotations induced by amphotericin but also significantly reduced the damage to the substantia nigra (69% of dopamine cells compared to the control group, normal cellular morphology) and the ventral tegmental area (Dopamine cells increased by 60%, cell morphology returned to normal); and, apomorphine significantly weakened dopamine dopamine damage striatum 6-hydroxydopamine, DOPAC / DA rate returned to normal. Conclusion Apomorphine not only has neuroprotective effect but also has neurotrophic effects in striatum of 6-OHDA-induced striatum. However, neurotrophic effects are confined to the ventral tegmental area and increase with time.