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对由绿色木霉(Trichoderma viride A 10)菌株的粗酶粉中分离纯化得到的17个内切葡聚糖酶的某些物理、化学性质和对5种纤维性材料的水解作用进行了研究,当它们分别作用于5种结晶度和聚合度不同的纤维性材料时,表现的比活力值是高度相关的,纤维材料的物理性质对酶的比活力有重要影响.在葡聚糖纤维二糖水解酶协同下,它们都能水解棉纤维但当它们共同作用于棉纤维素时,却只有9个组分的主效应是显著的.进一步的研究表明,酶的浓度效应在各组分间的协同作用中有重要影响.对纤维素酶解利用研究的策略提出了讨论.
Some physical and chemical properties of 17 endoglucanases isolated and purified from the crude enzyme powder of Trichoderma viride A 10 were studied and the hydrolysis of 5 kinds of fibrous materials was studied. When they act on five kinds of fibrous materials with different degrees of crystallinity and degree of polymerization, respectively, the specific activity value is highly correlated, and the physical properties of the fiber material have a significant impact on the specific activity of the enzyme.In glucan cellobiose They all hydrolyzed cotton fiber, but when they co-act on cotton fiber, only 9 main effects were significant.Further study showed that the effect of enzyme concentration between the various components Synergies have an important influence on the utilization of cellulase research proposed a strategy.