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目的：观察脑缺血再灌注中线粒体钙（MitochondriaCaloium,MCa）、钙调素（Calmodulin,CaM）、兴奋性氨基酸（ExcitatoryAminoAcid,EAA）、丙二醛（Malondiadehyde,MDA）的动态变化,研究探索其变化时相,为阻抑其自稳平衡紊乱的发生提供科学的时间效应点。方法：采用大鼠全脑缺血再灌注模型（4VO）,测定假手术组、缺血30min再灌注1h、6h、12h组大脑皮层、海马组织MCa、CaM、EAA、MDA的含量。结果：缺血30min再灌注1h鼠大脑皮层、海马组织MCa、CaM、MDA含量显著升高，EAA含量显著降低，再灌注6h后，MCa、CaM继续升高，EAA、MDA回复到假手术组水平,当再灌注到12h时．MCa、CaM也相继回复到假手术组水平。结论：脑缺血再灌注早期（1h），Ca++、EAA、氧自由基自稳平衡紊乱发生，Ca++自稳平衡紊乱回复较EAA、氧自由基晚。
OBJECTIVE: To observe the dynamic changes of mitochondrial calcium (MCa), calmodulin (CaM), excitatory amino acid (EAA) and malondiadehyde (MDA) in rats with cerebral ischemia-reperfusion injury Change the phase, in order to suppress the occurrence of self-stabilizing equilibrium disorder to provide scientific time effect point. Methods: The content of MCa, CaM, EAA and MDA in cerebral cortex and hippocampus of rats in sham-operated group, ischemia-reperfusion group 1h, 6h, 12h were measured by using rat model of global cerebral ischemia-reperfusion. Results: The levels of MCa, CaM and MDA in cerebral cortex and hippocampus of rats were significantly increased at 30 min after reperfusion for 1 h, and the levels of EAA were significantly decreased. After reperfusion for 6 h, the levels of MCa and CaM increased and the levels of EAA and MDA returned to the sham group , When reperfusion to 12h. MCa, CaM also returned to the sham group level. Conclusion: In the early stage of cerebral ischemia-reperfusion (1h), Ca ++, EAA, oxygen free radical instability disorder occurs, Ca ++ homeostasis disorder recovery than EAA, oxygen free radicals later.