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目的:观察兰州地区慢性乙型肝炎病毒基因型与干扰素治疗的关系。方法:用套式PCR方法扩增69例经干扰素治疗的慢性乙型肝炎病人的S基因,利用限制性内切酶Sty I和Bsr I平行酶切鉴别乙型肝炎病毒基因型,并追踪其治疗效果。结果:B型19例(27.5％),C型35例(50.7％),D型12例(17.3％)。对于干扰素治疗的应答率,B型与C、D型有显著性差异(p<0.05),C型与D型无显著性差异(P>0.05)。结论:B型病人对干扰素治疗有较好的应答,HBV基因型可能是预测干扰素治疗的因素之一。
Objective: To observe the relationship between chronic hepatitis B virus genotypes and interferon therapy in Lanzhou area. Methods: S gene of 69 patients with chronic hepatitis B treated by interferon was amplified by nested PCR method. The hepatitis B virus genotypes were identified by restriction enzyme digestion with Sty Ⅰ and Bsr I. treatment effect. Results: There were 19 cases (27.5%) in type B, 35 cases (50.7%) in type C and 12 cases (17.3%) in type D. The response rate to interferon therapy was significantly different between type B and C, D (p <0.05), while there was no significant difference between type C and type D (P> 0.05). Conclusion: Patients with type B have a better response to interferon therapy. HBV genotype may be one of the predictors of interferon therapy.