A Non-Marker Mutagenesis Strategy to Generate Poly-hrp Gene Mutants in the Rice Pathogen Xanthomonas

• 作 者: ZOU Li-fang LI Yu-rong CHEN Go
• 机 构: School of Agriculture and Biology,Key Laboratory of Monitoring and Management for Plant Diseases and
• 出 处: 中国农业科学（英文版）
• 发表日期: 2004年期
• 关键词: Xanthomonas oryzae pv. oryzicola suicide vector knockout mutagenesis hrp gene

Xanthomonas oryzae pv.oryzicola (Xoc),the critical pathogen causing bacterial leaf streak in rice,possesses a hrp cluster that is responsible for triggering hypersensitive response (HR) in non-host tobacco and pathogenicity in host rice,and is considered to be one of the model pathogens in the rice model plant.Here,we developed a high-throughput mutagenesis system using a two-step integration mediated by a novel suicide vector pKMS1.It was used to generate single or poly-gene mutants of hpa1,hpa2,hrcV,hrpE,hpaB,and hrpF gene for functional analysis.In total,five single,four double,and two triple hrp gene mutants were constructed.The double and triple hrp gene deletion mutants triggered novel phenotypes in planta.Our data suggest that pKMS1 is a useful tool for non-marker mutagenesis of multiple genes in Xoc.