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OBJECTIVE The aim of the study was to examine the reversal effects of ultrasound (US)on the MDR in HepG2/ADM,a HepG2 cell line resistant on Adriamycin(ADM),and to study the mechanism of US action.METHODS Using the MTT assay, the effects of US on MDR in HepG2/ADR cells were studied.Before and after the treatment with 0.5W/cm2 low intensity ultrasound(LIUS),the expression of the MDR-related genes,mdr1,mrp and lrp was assayed with the reverse transcriptase ploymerase chain reaction(RT-PCR)and the levels of their respective protein expression determined by flow cytometry.By usin confocal laser scanning microscopy(CLSM), we examined the intracellular daunorubicin(DNR)distribution,and the effects on the cells of treatment with US or DNR.RESULIS LIUS significantly reversed MDR in HepG2/ADR cells. After treatment with LIUS at 0.5W/cm2,chemosensitivity to ADM and DNR increased 3.35-fold and 2.81-fold,respectively.The reversal activity by LIUS plus verapamil(VER)was stronger than with either US or VER alone.After trea ment with 0.5W/cm2, the expression of both the MDR1 and the MRP mRNA genes began to declin(P<0.01 and P<0.05,respectively);the expression ofLRP showed no significant changes.Changes in the wxpression of the P-glycoprotein(P-gp)and MRP were similar to those of their mRNA expression.Results of the CLSM showed that administration of US(0.5W/cm2)or VER (15.7μM)with DNR to HepG2/ADM cells showed a significant change in the distribution of DNR in the cells.CONCLUSION Our results show that LIUS can reverse MDR.The reversl effects are stronger than those of either US or VER alone,when combined with VER administration.As LIUS is noninvasive casuing no toxicity,it might have potential for clinical application.The reversal mechanism needs further study.