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对转 Bt基因抗虫杂交棉正、反交 F1代与抗虫亲本的 Bt蛋白表达量及抗棉铃虫差异性研究表明 :抗虫亲本与其杂种 F1代均高抗棉铃虫 ,但抗虫亲本的抗虫性略好于其杂种 F1代 ,并且明显地高于非抗虫亲本 ;正、反交杂种 F1代间的抗虫性几乎没有差异。生长前期的抗虫性好于后期 ,同一时期嫩叶或侧枝生长点的抗虫性好于幼蕾。抗虫亲本叶片和花瓣的 Bt蛋白含量明显地高于其杂种 F1代 ,抗虫亲本功能叶的 Bt蛋白含量明显地高于其上部非功能叶 ,而杂种 F1代功能叶的 Bt蛋白含量则明显地低于其上部非功能叶。盛花期后至吐絮期前 ,叶片和花瓣的 Bt蛋白表达量明显增加 ,在抗虫亲本中表现最为明显。与叶片相比 ,在花瓣中检测到的 Bt蛋白含量极低。正、反交 F1代间的 Bt蛋白表达量差异较小或无规律可循
The results of Bt protein expression and resistance to bollworm in positive and negative crossbreed F1 progeny and insect-resistant parents of Bt transgenic cotton showed that the resistant parents and their F1 hybrids were highly resistant to bollworm, Insect resistance was slightly better than that of hybrid F1, and was significantly higher than that of non-insect-resistant parents; there was almost no difference in insect resistance between F1 and F1 hybrids. Pest resistance of the early growth stage is better than that of the late stage, and the resistance of young leaves or collateral growth points in the same period is better than that of young buds. The Bt protein content in leaves and petals of the insect-resistant parents was significantly higher than that in the F1 hybrids. The Bt protein content of the insect-resistant parents was significantly higher than that of the upper non-function leaves, while the Bt protein content of the functional leaves of the F1 hybrids was significantly Lower than the upper non-functional leaves. Bt protein expression in leaves and petals increased significantly after flowering to boll opening, and was most obvious in insect-resistant parents. The Bt protein content detected in the petals is extremely low compared to the leaves. Positive and negative F1 generation Bt protein expression between the small or no law to follow