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本文报导了由一名前往加纳、象牙海岸的旅行者带入德国的新拉沙病毒株的分离及特征。该病毒株被命名为“AV”,最早发现于西非,以前此地区没有拉沙热的报导。研究者将该患者血清中分离出的病毒进行了扩增和测序,采用长范围逆转录PCR扩增了S RNA的全长(3.4kb)。结果表明AV株的编码序列与所有已知的拉沙典型株(Josiah,Nigeria和LP)约有20％的不同,主要是在第三密码区。研究者认为目前已了解到了Nigeria、Josiah和AV这三种拉沙病毒株的全部S RAN,从它们全长序列表现出的较大差异看,拉沙病毒是由一种一元、遗传多态群组成。从种系遗传学角度看AV株最接
This article reports the isolation and characterization of a new Lhasa strain that was brought into Germany by a traveler to Ghana and the Ivory Coast. The strain was named “AV” and was first discovered in West Africa, where there was no previous report of Lassa fever. The researchers amplified and sequenced the virus isolated from the patient’s serum and amplified the full length of the S RNA (3.4 kb) using long-range reverse transcription PCR. The results show that the coding sequence of the AV strain is about 20% different from that of all known Lhasa typical strains (Josiah, Nigeria and LP), mainly in the third coding region. The researchers believe that the entire S RAN of Nigeria, Josiah and AV are now known, and from the large differences in their full-length sequences, the Lassa virus is composed of a single, composition. AV strains from the perspective of germ-line genetic study the most