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目的:研究β-胡萝卜素减轻阿霉素所致的大鼠心脏毒性的作用及其机制,方法:应用光学显微镜技术观察心肌组织的病理变化,心肌MDA值用巴比妥酸法测定,SOD活性用邻苯三酚法测定,GSH-Px活性用DTNB法测定,运用顺磁共振(ESR)技术测定半醌自由基。结果:β-胡萝卜素10或30mg·kg~(-1)可明显减轻阿霉素引起的大鼠心肌损害,保护SOD,GSH-Px活性,对抗阿霉素引起的心肌MDA水平升高,体外实验表明,β-胡萝卜素可以清除阿霉素诱导产生的半醌自由基,在0.02,0.1,1.0mmol·L~(-1)三个浓度时的抑制率分别为47.7％,76.6％,85.2％。结论:β-胡萝卜可以减轻阿霉素引起的大鼠心肌损害,其机制与抗脂质过氧化和清除半醌自由基有关。
OBJECTIVE: To study the effect and mechanism of β-carotene on doxorubicin-induced cardiotoxicity in rats. Methods: The pathological changes of myocardium were observed by light microscopy. The activity of MDA was measured by barbituric acid method and SOD activity Using pyrogallol method, GSH-Px activity was measured by DTNB method and paraquat free radical was determined by paramagnetic resonance (ESR) technique. Results: β-carotene 10 or 30 mg · kg -1 could obviously alleviate the myocardial damage caused by doxorubicin, protect the activities of SOD and GSH-Px, increase the myocardial MDA level induced by doxorubicin, Experiments show that β-carotene can eliminate doxorubicin induced semiquinone free radicals generated at the concentrations of 0.02,0.1,1.0mmol·L -1 (47.7%, 76.6%, 85.2 %. Conclusion: β-carotene can attenuate doxorubicin-induced myocardial damage in rats. The mechanism is related to anti-lipid peroxidation and scavenging semiquinone free radicals.