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目的:比较分光光度法和吸附质量法测定鞣质含量的区别,并对现行药典收载的分光光度法进行改进,为完善和优化鞣质检测方法提供参考。方法:以大黄为研究对象,比较现行的分光光度法和经典的吸附质量法检测鞣质含量的差异;参考并比较欧洲药典、《中华人民共和国药典》以及我国食品检测国家标准收载的鞣质含量测定方法,对现行《中华人民共和国药典》收载的方法进行改进。结果:现行的分光光度法与皮粉吸附质量法测定的大黄鞣质含量结果差别显著,前者的结果比后者小2~3倍。结论:改进后的分光光度法采用鞣酸为对照品,超声法进行提取,干酪素为吸附剂,测定结果与经典的皮粉吸附质量法较为接近,同时缩短了检测时间。
OBJECTIVE: To compare the difference between the content of tannins measured by spectrophotometry and adsorption mass spectrometry, and to improve the spectrophotometry included in the current Pharmacopoeia to provide reference for improving and optimizing tannin detection methods. Methods: The rhubarb was used as the research object, and the differences between the current tannin content and the traditional methods of spectrophotometry and classical adsorption quality were compared. The enamel contained in the European Pharmacopoeia, the Pharmacopoeia of the People’s Republic of China and the national standards for food testing were compared and compared. The method of content determination is to improve the methods currently contained in the “People’s Republic of China Pharmacopoeia”. RESULTS: There was a significant difference between the results obtained by the current spectrophotometric method and the quality of P. trachomata extract method. The former was 2 to 3 times smaller than the latter. Conclusion: The improved spectrophotometric method uses tannic acid as the reference substance and ultrasonic extraction. Casein is the adsorbent. The results are close to those of the classical skin powder adsorption quality method, and the detection time is shortened.