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目的：为放射免疫显像在诊断食管癌淋巴结转移中的应用，提供基础研究支持。方法：①免疫组化(LSAB法)测定不同组织冷冻切片和抗人食管鳞癌单抗G9的反应；②125I标记G9，形成标记化合物，在荷食管癌裸鼠腹腔注射125I-G9后的连续3天内，测定各主要组织、器官的放射性。结果：①LSAB显示，食管癌原发灶和转移淋巴结呈阳性反应，正常食管和无转移淋巴结阴性。②125I-G9在裸鼠体内分布显示，肿瘤组织的放射性计数均明显高于其它器官/组织；各器官/组织的T/NT值在2～7之间；放射自显影的结果也显示，肿瘤部位有大量的放射性浓集。结论：①单抗G9能选择性定位于食管癌原发灶与转移淋巴结的癌细胞胞膜。②125I-G9在荷食管癌裸鼠有肿瘤组织导向作用，显像效果良好，提示放射免疫显像在食管癌转移淋巴结定位，有潜在的临床应用前景。
Objective: To provide basic research support for the application of radioimmunoimaging in the diagnosis of lymph node metastasis of esophageal cancer. Methods: ① The immunohistochemistry (LSAB method) was used to measure the reaction of frozen sections of different tissues and G9 monoclonal antibody against human esophageal squamous cell carcinoma; ②125I labeled G9 to form the labeled compound. After continuous intraperitoneal injection of 125I-G9 Days, the determination of the major organizations, organs of radioactivity. Results: (1) LSAB showed that the primary esophageal cancer and metastatic lymph nodes were positive, and the normal esophagus and non-metastatic lymph nodes were negative. The distribution of 125I-G9 in nude mice showed that the radioactivity counts of tumor tissues were significantly higher than those of other organs / tissues. The T / NT values of various organs / tissues ranged from 2 to 7. The results of autoradiography also showed that tumor sites There is a lot of radioactive concentration. Conclusion: ① McAb G9 can selectively locate in the esophageal cancer metastasis and lymph node metastasis of cancer cell membrane. ②125I-G9 in tumor-bearing nude mice with tumor-oriented role, imaging effect is good, suggesting that radioimmunoimaging metastasis in esophageal cancer lymph node mapping, there is potential for clinical application.