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暴发型乙型肝炎的发病机制尚不清楚,可能是 病毒和宿主的免疫应答同时参与所致。以往研究发现,慢性乙型肝炎患者肝组织Fas抗原过度表达,并且这种表达与肝炎的活动性有关。本文研究Fas系统在暴发型乙型肝炎中的作用和HBV感染的病毒学特征。 研究对象为暴发型乙型肝炎3例:用斑点杂交法和聚合酶链反应测得HBV DNA;分析S和C基因的序列;用酶免疫法测得血清中可溶性Fas抗原水平;TUNEL技术测定细胞凋亡;用免疫过氧化酶法评估Fas抗原表达。同时,以10名健康人作对
The pathogenesis of fulminant hepatitis B is not yet clear, probably due to the simultaneous involvement of both the virus and the host’s immune response. Previous studies have found that patients with chronic hepatitis B liver tissue Fas antigen overexpression, and this expression and hepatitis activity. This article studies the role of Fas system in fulminant hepatitis B and the virological characteristics of HBV infection. The subjects were fulminant hepatitis B in 3 cases: HBV DNA was detected by dot blot hybridization and polymerase chain reaction; the sequences of S and C genes were analyzed; the level of soluble Fas antigen in serum was measured by enzyme immunoassay; TUNEL assay Apoptosis; Fas antigen expression was assessed by immunoperoxidase. At the same time, to 10 healthy people against